Application
Western blot analysis of OC-3 expression in Hep G2 whole cell lysate.| Product Name | OC-3 Antibody (W-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of OC-3 of human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | OC-3 (One cut domain family member 3) is a 494 amino acid nuclear protein that acts as a transcriptional activator. OC-3 binds the consensus DNA sequence 5’-DHWATTGAYTWWD-3' on a diverse array of gene promoters, such as Prealbumin and HNF-3∫. OC-3 is a member of the ONECUT homeobox family and contains one CUT DNA-binding domain and one homeobox DNA-binding domain. ONECUT proteins regulate gene networks by controlling transcription factor expression and also play an important role in cell metabolism and differentiation. OC-3 shares a significant amount of sequence similarity with OC-1 and OC-2 and is found in upper intestine, stomach and brain in both adult and embryonic mouse. Due to their expression patterns, it is likely that all ONECUT proteins participate in the regulation of organ development from the foregut and midgut endoderm. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of OC-3 of human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of OC-3 of human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 390874 |
|---|
Application
Western blot analysis of OC-3 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
