Application
Western blot analysis of ERG25 expression in human fetal liver tissue extract.| Product Name | ERG25 Antibody (Y-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of ERG25 of mouse and human origin by WB, IP and ELISA |
| Modification Notes | Belonging to the sterol desaturase family, ERG25, also known as C-4 methylsterol oxidase or Methylsterol monooxygenase, is a 293 amino acid enzyme that performs the first of three enzymatic steps required to remove the two methyl groups leading to cholesterol. ERG25 is a multi-pass membrane protein that resides within the plasma membrane and the membrane of the endoplasmic reticulum. Concentrations of ERG25 mRNA increase when subjected to low iron growth conditions, suggesting that the enzyme is not regulated by iron but by an end product of the ergosterol pathway. Low-density lipoprotein (LDL) downregulates ERG25 expression in the vascular wall, an event that may be inhibited via SREBP overexpression. ERG25 is encoded by a gene located on human chromosome 4q32.3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ERG25 of mouse and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of ERG25 of mouse and human origin by WB, IP and ELISA |
| Entrez Gene | 6307 |
|---|
Application
Western blot analysis of ERG25 expression in human fetal liver tissue extract.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
