Application
Western blot analysis of Calmegin expression in Hep G2 whole cell lysate.| Product Name | Calmegin Antibody (V-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Calmegin of human origin by WB, IP and ELISA |
| Modification Notes | Calmegin, belonging to the calreticulin family, is expressed in testis as an endoplasmic reticulum membrane protein, where it acts as a chaperone protein and plays a role in spermatogenesis. Calmegin is a single-pass type I membrane protein that is transcriptionally regulated in coordination by CpG methyltransferase and histone deacetylase (HDAC). First expressed in meiotic prophase of spermatocytes, Calmegin facilitates sperm-egg zona pellicuda binding through association with sperm membrane proteins (fertilin alpha and beta). A loss in Calmegin results in male sterility. However, if the zona pellicuda is partially dissected and fertilized in vitro, the egg will develop normally. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Calmegin of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Calmegin of human origin by WB, IP and ELISA |
| Entrez Gene | 1047 |
|---|
Application
Western blot analysis of Calmegin expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
