Application
Western blot analysis of human Peroxin 10 transfected 293T whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human muscle tissue showing cytoplasmic localization.| Product Name | Peroxin 10 Antibody (F-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Peroxin 10 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Peroxisomes are single-membrane bound organelles present in virtually all eukaryotic cells. They are involved in numerous catabolic and anabolic pathways, including beta-oxidation of very long chain fatty acids, metabolism of hydrogen peroxide, plasmalogen biosynthesis and bile acid synthesis. The Peroxin gene family, which includes more than 20 members, is required for peroxisome biogenesis. Peroxin 10 (Peroxisome biogenesis factor 10), also known as RNF69 (RING finger protein 69), is a 326 amino acid protein that exhibits E3 ligase activity in vitro, suggesting that it is involved in Ubc4-dependent ubiquination. Defects in the gene encoding Peroxin 10 are the result of a number of different disorders, such as Peroxisome biogenesis disorder complementation group 7, Zellweger syndrome and adrenoleukodystrophy neonatal. There are two isoforms of Peroxin 10 that are produced as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Peroxin 10 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Peroxin 10 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 5192 |
|---|
Application
Western blot analysis of human Peroxin 10 transfected 293T whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human muscle tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
