Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in seminiferous ducts.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Ero1-Lα expression in non-transfected: (A) and mouse Ero1-Lα transfected: (B) 293T whole cell lysates and mouse ovary tissue extract (C).Application
Western blot analysis of Ero1-Lα expression in HEK293 (A) and HeLa (B) whole cell lysates.| Product Name | Ero1-Lα Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 91-180 mapping near the N-terminus of Ero1-Lα of human origin |
| Modification Notes | Ero1-La (endoplasmic oxidoreductin-1-like), also known as Ero1a or oxidoreductin-1-La, is an essential oxidoreductase that oxidizes proteins and is required for the folding of immunoglobulins. Ero-1La covalently binds with PDI (protein disulfide-isomerase) and together they produce disulfide bonds between proteins in the endoplasmic reticulum. Ero1-La and SIRT1 regulate adiponectin secretion from adipose tissue. Ero1-La and associated proteins also modulate PPARg (peroxisome proliferator-activated receptor g) and SIRT1 activities. Ero1-La is stimulated by hypoxia, suggesting that it is regulated through the HIF (hypoxia inducible transcrip-tion factor) pathway. Ero1-La is ubiquitously expressed at low levels but expressed at high levels in upper digestive tract and esophagus. Ero1-La may function both as a monomer and a homodimer. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Ero1-Lα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 91-180 mapping near the N-terminus of Ero1-L&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 91-180 mapping near the N-terminus of Ero1-Lα of human origin |
| Entrez Gene | 30001 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in seminiferous ducts.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Ero1-Lα expression in non-transfected: (A) and mouse Ero1-Lα transfected: (B) 293T whole cell lysates and mouse ovary tissue extract (C).
Application
Western blot analysis of Ero1-Lα expression in HEK293 (A) and HeLa (B) whole cell lysates.| Application Notes | recommended for detection of Ero1-Lα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
