Application
Western blot analysis of mt-TyrRS expression in CEM whole cell lysate.| Product Name | mt-TyrRS Antibody (C-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of mt-TyrRS of human origin by WB and ELISA |
| Modification Notes | The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. Aminoacyl-tRNA synthetases function to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. Mt-TyrRS (Tyrosyl-tRNA synthetase, mitochondrial), also known as Tyrosine-tRNA ligase and Tyrosal-tRNA synthetase 2, is a 477 amino acid protein that belongs to the class-I aminoacyl-tRNA synthetase family. Containing a 16-amino acid mitchondrial targeting signal, mt-TyrRS is localized to the mitochondrial matrix where it exists as a homodimer and functions primarily to catalyze the attachment of tyrosine to tRNA(Tyr) in a two-step reaction. First, tyrosine is activated by ATP to form Tyr-AMP, then it is transferred to the acceptor end of tRNA(Tyr). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBELISA |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of mt-TyrRS of human origin by WB and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of mt-TyrRS of human origin by WB and ELISA |
| Entrez Gene | 51067 |
|---|
Application
Western blot analysis of mt-TyrRS expression in CEM whole cell lysate.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
