Application
Western blot analysis of Myosin Ic expression in K-562 whole cell lysate.Application
Western blot analysis of Myosin Ic expression in 293T whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human carcinoma tissue showing cytoplasmic staining.| Product Name | Myosin Ic Antibody (C-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Myosin Ic of human origin by WB, IP, IF and IHC(P) |
| Modification Notes | Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Troponin facilitates interaction between actin and myosin by binding to Ca2+. Troponin is made up of at least two subunits, which are divergent in cardiac muscle, fast skeletal muscle and slow skeletal muscle. Myosin is a hexamer of 2 heavy chains (MHC) and 4 light chains (MLC) that interacts with actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Myosin Ic (MYO1C) is also designated Myosin I beta. In vestibular hair cells, myosin-1c may be important for fast adaptation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Myosin Ic of human origin by WB IP IF and IHC(P) |
|---|---|
| Tissue Specificity | recommended for detection of Myosin Ic of human origin by WB, IP, IF and IHC(P) |
| Entrez Gene | 4641 |
|---|
Application
Western blot analysis of Myosin Ic expression in K-562 whole cell lysate.
Application
Western blot analysis of Myosin Ic expression in 293T whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human carcinoma tissue showing cytoplasmic staining.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
