Application
Western blot analysis of Eps15R expression in Y79 whole cell lysate.Application
Western blot analysis of Eps15L1 expression in non-transfected: (A) and human Eps15L1 transfected: (B) 293T whole cell lysates.| Product Name | Eps15R Antibody (N-16) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against a peptide mapping near the N-terminus of Eps15R of human origin |
| Modification Notes | Elucidation of the mechanism by which receptor tyrosine kinases (RTKs) modulate cellular physiology in response to stimuli is critical to the understanding of growth regulation, as miscues in RTK signaling can result in cellular transformation events that may ultimately lead to cancer. Eps15 (EGF-receptor pathway substrate 15) is an EGF receptor substrate that become become tyrosine phosphorylated subsequent to EGF stimulation. Over expression of Eps15 in NIH/3T3 cells causes cellular transformation, implying involvement of Eps15 in the regulation of cell proliferation. Eps15R (Eps15-related), also known as Eps15L1 (epidermal growth factor receptor substrate 15-like 1), is an 864 amino acid protein that contains one EF-hand domain and three EH domains. Localized to a variety of places within the cell, including the nucleus, cytoplasm and membrane-coated pits, Eps15R is thought to function as a component of clathrin-coated pits. Like Eps15, Eps15R may play an important role in receptor-mediated endocytosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| recommended for detection of Eps15R of human origin by WB, IP and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against a peptide mapping near the N-terminus of Eps15R of human origin |
|---|---|
| Tissue Specificity | raised against a peptide mapping near the N-terminus of Eps15R of human origin |
| Entrez Gene | 58513 |
|---|
Application
Western blot analysis of Eps15R expression in Y79 whole cell lysate.
Application
Western blot analysis of Eps15L1 expression in non-transfected: (A) and human Eps15L1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Eps15R of human origin by WB, IP and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
