Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing staining of intercalated disks.Application
Western blot analysis of DREAM expression in non-transfected: (A) and human DREAM transfected: (B) 293T whole cell lysates.Application
Western blot analysis of DREAM expression in non-transfected: (A) and human DREAM transfected: (B) 293T whole cell lysates.Application
Western blot analysis of DREAM expression in non-transfected: (A) and mouse DREAM transfected: (B) 293T whole cell lysates.Application
Western blot analysis of DREAM expression in non-transfected: (A) and human DREAM transfected: (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human malignant melanoma tissue showing membrane staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of DREAM expression in H4 whole cell lysate.Application
Western blot analysis of human recombinant DREAM fusion protein.| Product Name | DREAM Antibody (FL-214) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding amino acids 1-214 representing full length DREAM of human origin |
| Modification Notes | DREAM (for DRE-antagonist modulator) is a Ca2+-regulated transcriptional repressor that specifically binds to the downstream regulatory elements (DRE). DRE is a regulatory sequence that silences basal transcription and is localized to the promoter region of the gene encoding human prodynorphin, an opioid peptide involved in memory acquisition and pain. DREAM forms functional homotetramers that are required for the interaction with the DRE. This association is highly influenced by calcium, as an increase in Ca2+ directly inhibits DREAM binding and thereby blocks the repressor activity of DREAM. DREAM transcripts are detected in brain, thymus and thyroid gland, and it is expressed as a nuclear protein. DREAM has been shown to inhibit transcription of other proteins containing DRE-like motifs, including the gene encoding for the AP-1 transcription factor c-Fos, suggesting that DREAM may influence a wide variety of cellular genes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of DREAM of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding amino acids 1-214 representing full length DREAM of human origin |
|---|---|
| Tissue Specificity | epitope corresponding amino acids 1-214 representing full length DREAM of human origin |
| Entrez Gene | 30818 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing staining of intercalated disks.
Application
Western blot analysis of DREAM expression in non-transfected: (A) and human DREAM transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of DREAM expression in non-transfected: (A) and human DREAM transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of DREAM expression in non-transfected: (A) and mouse DREAM transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of DREAM expression in non-transfected: (A) and human DREAM transfected: (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human malignant melanoma tissue showing membrane staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of DREAM expression in H4 whole cell lysate.
Application
Western blot analysis of human recombinant DREAM fusion protein.| Application Notes | recommended for detection of DREAM of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
