Application
Western blot analysis of RCL1 expression in mouse brain (A) and Hep G2 (B) tissue extracts.| Product Name | RCL1 Antibody (C-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of RCL1 of mouse and human origin by WB, IP and ELISA |
| Modification Notes | RCL1 (RNA terminal phosphate cyclase-like 1), also known as RNAC, RPC2, RPCL1 or RTC2, is a 373 amino acid protein that localizes to the nucleolus and belongs to the type 2 subfamily of RNA 3’-terminal cyclases. Involved in the proper maturation of the small 18S ribosomal subunit, RCL1 plays a role in the biogenesis of the 40S ribosomal subunit, specifically in early pre-rRNA processing steps at the A0, A1 and A2 40S cleavage sites. Processing at the 40S cleavage sites is crucial for correct 18S RNA formation. Human RCL1 shares 97% sequence identity with its mouse counterpart, suggesting a conserved role between species. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of RCL1 of mouse and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of RCL1 of mouse and human origin by WB, IP and ELISA |
| Entrez Gene | 10171 |
|---|
Application
Western blot analysis of RCL1 expression in mouse brain (A) and Hep G2 (B) tissue extracts.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
