Application
Western blot analysis of MYCBP expression in non-transfected 293T: (A), human MYCBP transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of MYCBP expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining.| Product Name | MYCBP Antibody (R-17) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against MYCBP of human origin |
| Modification Notes | The c-Myc proto-oncogene plays a significant role in cell proliferation, differentiation, transformation and apoptosis. A novel c-Myc binding protein MYCBP (also designated AMY-1) binds to the transactivation domain of c-Myc and stimulates the activation of E-box-dependent transcription. MYCBP translocates from the cytoplasm to the nucleus during S phase when increased expression of c-Myc occurs. MYCBP has also been shown to associate with AKAP 149 and AKAP 84 in mitochondria of somatic cells and sperm, which suggests a role for MYCBP in spermatogenesis. In addition, MYCBP localizes to the tran-Golgi network (TGN) and the nucleus, where it binds the high molecular weight guanine-nucleotide exchange factor BIG2 in vivo to coordinate ADP-ribosylation factor-mediated membrane trafficking and signaling pathways. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of MYCBP of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against MYCBP of human origin |
|---|---|
| Tissue Specificity | raised against MYCBP of human origin |
| Entrez Gene | 26292 |
|---|
Application
Western blot analysis of MYCBP expression in non-transfected 293T: (A), human MYCBP transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of MYCBP expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining.| Application Notes | recommended for detection of MYCBP of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
