Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis showing nuclear staining of cells in seminiferus ducts at low magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis showing nuclear staining of cells in seminiferus ducts at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of ZWINT expression in non-transfected: (A) and mouse ZWINT transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ZWINT expression in non-transfected 293T: (A), human ZWINT transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Western blot analysis of ZWINT expression in non-transfected: (A) and human ZWINT transfected: (B) 293 whole cell lysates.| Product Name | ZWINT Antibody (FL-277) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-277 (deletion 228-229) representing full length ZWINT of human origin |
| Modification Notes | ZWINT (ZW10 interactor), also known as KNTC2AP or HZwint-1, is a 277 amino acid protein that is involved in kinetochore function. Localized to the cytoplasm during interphase and to kinetochores from late prophase to anaphase, ZWINT interacts with ZW10 (Zeste White 10) and functions to regulate the association between ZW10 and kinetochores. Additionally, ZWINT is part of a kinetochore complex composed of proteins such as MIS12 (MIND kinetochore complex component) and PMF-1 (polyamine-modulated factor 1) that work in concert to ensure proper kinetochore formation and spindle checkpoint activity. Defects in the gene encoding ZWINT are associated with the pathogenesis of Roberts syndrome, an autosomal recessive disorder characterized by growth retardation due to premature chromosome separation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of ZWINT of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-277 (deletion 228-229) representing full length ZWINT of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-277 (deletion 228-229) representing full length ZWINT of human origin |
| Entrez Gene | 11130 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis showing nuclear staining of cells in seminiferus ducts at low magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis showing nuclear staining of cells in seminiferus ducts at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of ZWINT expression in non-transfected: (A) and mouse ZWINT transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ZWINT expression in non-transfected 293T: (A), human ZWINT transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Western blot analysis of ZWINT expression in non-transfected: (A) and human ZWINT transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of ZWINT of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
