Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.Application
Antibodies tested include E2A (N-649): (A), E2A (Yae): (B) and E2A (V-18): (C,D).Application
Western blot analysis of E2A expression in Jurkat (A) and K-562 (B) whole cell lysates.| Product Name | E2A Antibody (N-649) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against amino acids 1-649 of E2A of human origin |
| Modification Notes | Transcription factor 3 (E47, E12, E2A immunoglobulin enhancer binding factors E12/E47, E2A, ITF1, TCF3) influences gene expression during B cell maturation.Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development includes Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes. Both homo- and hetero-oligomers of these proteins are able to distinguish very closely related E box proteins and are believed to play important roles in lineage-specific gene expression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of E2A isoforms E12 and E47 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 1-649 of E2A of human origin |
|---|---|
| Tissue Specificity | raised against amino acids 1-649 of E2A of human origin |
| Entrez Gene | 6929 |
|---|
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.
Application
Antibodies tested include E2A (N-649): (A), E2A (Yae): (B) and E2A (V-18): (C,D).
Application
Western blot analysis of E2A expression in Jurkat (A) and K-562 (B) whole cell lysates.| Application Notes | recommended for detection of E2A isoforms E12 and E47 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
