| Product Name | E2A Antibody (H-208) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against amino acids 208-649 mapping within a basic helix-loop-helix domain of E2A of human origin |
| Modification Notes | Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development includes Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. The E2A gene encodes two basic helix-loop-helix (bHLH) transcription factors, designated E12 and E47, produced by alternative splicing. E12 and E47 are nuclear proteins that form heterodimers with Myo D transcription factors and play a role in determining tissue-specific cell fate during embryogenesis. E12 and E47 bind to the kappa-E2 site in the kappa immunoglobulin gene enhancer. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of E2A isoforms E12 and E47 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 208-649 mapping within a basic helix-loop-helix domain of E2A of human origin |
|---|---|
| Tissue Specificity | raised against amino acids 208-649 mapping within a basic helix-loop-helix domain of E2A of human origin |
| Entrez Gene | 6929 |
|---|
| Application Notes | recommended for detection of E2A isoforms E12 and E47 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
