Application
Western blot analysis of Epac expression in non-transfected: (A) and human Epac transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Epac expression in non-transfected: (A) and human Epac transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Epac expression in non-transfected: (A) and human Epac transfected: (B) 293T whole cell lysates.Application
Western blot analysis of human recombinant Epac fusion protein.| Product Name | Epac Antibody (H-70) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 43-112 mapping at the N-terminus of Epac of human origin |
| Modification Notes | 3',5' cyclic adenosine monophosphate (cAMP)-regulated guanine nucleotide exchange factors Epac (Epac1, cAMP-GEFI) and Epac2 (cAMP-GEFII) activate the Ras family GTPases Rap 1 and Rap 2 by promoting GTP binding in a cAMP-dependent manner. Eukaryotic cAMP is a second messenger that induces physiological responses such as gene expression, growth, differentiation, secretion and neurotransmission. The human Epac gene maps to chromosome 12q13.11 with transcript being abundant in the kidney and heart. In situ hybridization indicates expression of Epac in adult rat brain and selective expression in neonatal brain, including septum and thalamus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Epac of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 43-112 mapping at the N-terminus of Epac of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 43-112 mapping at the N-terminus of Epac of human origin |
| Entrez Gene | 10411 |
|---|
Application
Western blot analysis of Epac expression in non-transfected: (A) and human Epac transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Epac expression in non-transfected: (A) and human Epac transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Epac expression in non-transfected: (A) and human Epac transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of human recombinant Epac fusion protein.| Application Notes | recommended for detection of Epac of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
