Application
Western blot analysis of p21 expression in untreated (A) and Novobiocin Sodium (U-6591) treated (B) T-47D whole cell lysates. Note down regulation of p21 expression in lane B.Application
Western blot analysis of p21 expression in HeLa nuclear extract.Application
Immunofluorescence staining of methanol-fixed C32 cells showing nuclear staining.Application
Antibodies tested include p21 (H-164): (A) and p21 (C-19)-G: (B).| Product Name | p21 Antibody (H-164) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-164 representing full length p21 of human origin |
| Modification Notes | It is now well established that cyclins play a positive role in promoting cell cycle transitions via their ability to associate with and activate their cognate cyclin-dependent kinases (Cdks). Cdk2 associates with cyclins A, D and E, and has been implicated in the control of the G1 to S phase transition in mammals. A novel Cdk-interacting protein, designated p21, Cip1 or WAF1, has been identified in cyclin A, cyclin D1, cyclin E and Cdk2 immunoprecipitates. p21 is a potent, tight-binding inhibitor of Cdks and can inhibit the phosphorylation of Rb by cyclin A-Cdk 2, cyclin E-Cdk2, cyclin D1-Cdk4 and cyclin D2-Cdk4 complexes. Expression of p21 is inducible by wildtype, but not mutant, p53. The mouse homolog of p21 is designated CAP20. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of p21 of mouse, rat and human origin by WB, IP, IF and ELISA partially cross-reactive with the related mitotic inhibitory protein, p27: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-164 representing full length p21 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-164 representing full length p21 of human origin |
| Entrez Gene | 1026 |
|---|
Application
Western blot analysis of p21 expression in untreated (A) and Novobiocin Sodium (U-6591) treated (B) T-47D whole cell lysates. Note down regulation of p21 expression in lane B.
Application
Western blot analysis of p21 expression in HeLa nuclear extract.
Application
Immunofluorescence staining of methanol-fixed C32 cells showing nuclear staining.
Application
Antibodies tested include p21 (H-164): (A) and p21 (C-19)-G: (B).| Application Notes | recommended for detection of p21 of mouse, rat and human origin by WB, IP, IF and ELISA partially cross-reactive with the related mitotic inhibitory protein, p27: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
