Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of Aminoacylase-1 expression in Caki-1 (A), K-562 (B) and HeLa (C) whole cell lysates and rat brain tissue extract (D) and human recombinant Aminoacylase-1 fusion protein (E).| Product Name | Aminoacylase-1 Antibody (H-144) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 265-408 mapping at the C-terminus of Aminoacylase-1 of human origin |
| Modification Notes | Aminoacylase-1, also designated N-acyl-L-amino-acid amidohydrolase or ACY-1, is a member of the largest metallopeptidase family called M20A. Aminoacylase-1 is a zinc-binding homodimeric enzyme expressed in kidney, brain, placenta and spleen. It is the most abundant of the aminoacylases. Aminoacylase-1 localizes to the cytoplasm and catalyzes the hydrolysis of N-acylated or N-acetylated amino acids. In addition, Aminoacylase-1 is a sphingosine kinase 1 (SphK1)-interacting protein and may also play a role in regulating responses of the cell to oxidative stress. The gene encoding Aminoacylase-1 is evolutionarily conserved in fish, frog, mouse, rat and human. Deficiency in the Aminoacylase-1 protein may result in defects of brain metabolism and function. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Aminoacylase-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 265-408 mapping at the C-terminus of Aminoacylase-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 265-408 mapping at the C-terminus of Aminoacylase-1 of human origin |
| Entrez Gene | 95 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of Aminoacylase-1 expression in Caki-1 (A), K-562 (B) and HeLa (C) whole cell lysates and rat brain tissue extract (D) and human recombinant Aminoacylase-1 fusion protein (E).| Application Notes | recommended for detection of Aminoacylase-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
