Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of SP-lyase expression in rat liver tissue extract.Application
Western blot analysis of SP-lyase expression in HUV-EC-C whole cell lysate.| Product Name | SP-lyase Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 131-430 mapping within a cytoplasmic domain of SP-lyase of human origin |
| Modification Notes | Sphingosine-1-phosphate lyase (SP-lyase) is a member of the group ll decarboxylase family that is involved in lipid metabolism. SP-lyase has a variety of functions contributing to normal development, including maintenance of the reproductive system, stress responses, tissue integrity and cell survival. Located in the membrane of the endoplasmic reticulum, SP-lyase is responsible for the irreversible degradation of sphingosine-1-phosphate (S1P). S1P is a lipid important in cell proliferation and migration and, once cleaved by SP-lyase, is degraded into fatty acids and phosphoethanolamine. Through its ability to regulate S1P expression, SP-lyase may play a role in stress-induced apoptosisand is thought to exhibit tumor suppressor activity by silencing S1P activity. Current research suggests that SP-lyase may be a useful target for cancer therapy drugs, as increasing its expression during tumorigenesis may help to regulate cell proliferation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SP-lyase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 131-430 mapping within a cytoplasmic domain of SP-lyase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 131-430 mapping within a cytoplasmic domain of SP-lyase of human origin |
| Entrez Gene | 8879 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of SP-lyase expression in rat liver tissue extract.
Application
Western blot analysis of SP-lyase expression in HUV-EC-C whole cell lysate.| Application Notes | recommended for detection of SP-lyase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
