Application
Western blot analysis of MacroH2A expression in human MacroH2A transfected: (A) and non-transfected: (B) 293 whole cell lysates.Application
Western blot analysis of human recombinant MacroH2A fusion protein.| Product Name | MacroH2A Antibody (H-39) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 144-182 mapping within an internal region of MacroH2A of human origin |
| Modification Notes | Eukaryotic histones are water soluble, basic nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer. The octamer consists of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. MacroH2A, also called core histone MacroH2A2 (mH2A2), is a variant Histone H2A, originally isolated in rat liver, that is nearly three times as large as conventional H2A. MacroH2A may be involved in stable X chromosome inactivation as it is enriched in inactive X chromosome chromatin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of MacroH2A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 144-182 mapping within an internal region of MacroH2A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 144-182 mapping within an internal region of MacroH2A of human origin |
| Entrez Gene | 55506 |
|---|
Application
Western blot analysis of MacroH2A expression in human MacroH2A transfected: (A) and non-transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of human recombinant MacroH2A fusion protein.| Application Notes | recommended for detection of MacroH2A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
