Application
Western blot analysis of karyopherin β1 expression in Jurkat whole cell lysate.Application
Western blot analysis of karyopherin β1 expression in 293T whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver tissue showing cytoplasmic staining.Application
Western blot analysis of karyopherin β1 expression in MDCK whole cell lysate.| Product Name | karyopherin β1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of karyopherin β1 of human origin |
| Modification Notes | Protein transport across the nucleus is a selective, multi-step process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Two cytosolic factors centrally involved in the recognition and docking process are the karyopherin a1 and karyopherin b1 subunits. Karyopherin a1 functions in the recognition and targeting of substrates destined for nuclear import, while karyopherin b1 serves as an adapter, tethering the karyopherin a1/substrate complex to docking proteins on the nuclear envelope, termed nucleoporins. Karyopherin a2 has been shown to complex with Epstein-Barr virus nuclear antigen 1 (EBNA-1). Certain RNA-binding proteins are imported to the nucleus by karyopherin b2, and karyopherin b3 appears to be involved in the import of some ribosomal proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of karyopherin β1 of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of karyopherin &beta 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of karyopherin β1 of human origin |
| Entrez Gene | 3837 |
|---|
Application
Western blot analysis of karyopherin β1 expression in Jurkat whole cell lysate.
Application
Western blot analysis of karyopherin β1 expression in 293T whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver tissue showing cytoplasmic staining.
Application
Western blot analysis of karyopherin β1 expression in MDCK whole cell lysate.| Application Notes | recommended for detection of karyopherin β1 of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
