Application
Western blot analysis of Nox3 expression in non-transfected: (A) and human Nox3 transfected: (B) 293T whole cell lysates.| Product Name | Nox3 Antibody (M-55) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 231-285 mapping within an extracellular domain of Nox3 of mouse origin |
| Modification Notes | Nox3 (GP91-3, NADPH oxidase 3) is a plasma membrane-associated enzyme that catalyzes the production of superoxide by a one-electron reduction of oxygen, using NADPH as the electron donor. Nox3 contains six membrane-spanning regions, conserved flavin and pyridine nucleotide-binding sites, and histidines possibly involved in heme ligation. It functions together with p22phox as an enzyme constitutively producing superoxide. Nox3 expression promotes p22phox transport to the plasma membrane and can be inhibited by mutations in the p22phox binding sites (SH3 domains) of p47phox or Nox1. Nox3 localizes to the vestibular and cochlear sensory epithelia and to spiral ganglions and participates in otoconia formation in inner ears, which is required for perception of balance and gravity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Nox3 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 231-285 mapping within an extracellular domain of Nox3 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 231-285 mapping within an extracellular domain of Nox3 of mouse origin |
| Entrez Gene | 50508 |
|---|
Application
Western blot analysis of Nox3 expression in non-transfected: (A) and human Nox3 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Nox3 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
