Application
Western blot analysis of PLC βb2 expression in rat PBL whole cell lysate.| Product Name | PLC β2 Antibody (H-255) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 821-1075 mapping within an internal region of PLC β2 of human origin |
| Modification Notes | Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC ∫1, PLC ∫2, PLC ∫3, PLC ∫4, PLC©1, PLC©2, PLC ∂1, PLC ∂2 and PLCe). PLC ∫s are the only PLC isforms that are regulated by G protein subunits and are activated by a heterotrimeric GTP-binding protein linked to various cell surface receptors. Two alternatively spliced forms (1181 and 1166 amino acids) of PLC ∫2 are generated in hematopoietic cells that differ in the carboxyl terminal sequence implicated in interaction of PLC ∫ enzymes with Gåq.The pleckstrin homology domain of PLC ∫2 is required for its targeting to the membrane and for substrate hydrolysis and its linker region exerts an inhibitory efect on basal PLC ∫2 activity. PLC ∫2 plays a major role in platelet activation and is mainly expressed in the periphery of the islet and acinar cells in rat pancreas. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PLC β2 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 821-1075 mapping within an internal region of PLC &beta 2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 821-1075 mapping within an internal region of PLC β2 of human origin |
| Entrez Gene | 5330 |
|---|
Application
Western blot analysis of PLC βb2 expression in rat PBL whole cell lysate.| Application Notes | recommended for detection of PLC β2 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
