Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Western blot analysis of TRPV6 expression in Hep G2 (A), Caco-2 (B), SW480 (C) and DU 145 (D) whole cell lysates.Application
Western blot analysis of TRPV6 expression in non-transfected: (A) and human TRPV6 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of TRPV6 expression in non-transfected 293T: (A), mouse TRPV6 transfected 293T: (B) and KNRK (C) whole cell lysates.Application
Western blot analysis of TRPV6 expression in Hep G2 (A), Caco-2 (B), SW480 (C), KNRK (D) and HCT-8 (E) whole cell lysates.| Product Name | TRPV6 Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 636-725 mapping at the C-terminus of TRPV6 of human origin |
| Modification Notes | The transient receptor potential (TRP) protein family consists of a diverse group of cation channels functioning in a variety of homeostatic and regulatory pathways. Four subfamilies exist, based on channel domain homology, not activating stimuli: C type (canonical or classical), V type (vanilloid receptor related), M type (melastatin related) and P type (PKD). TRPV6 belongs to the V type subfamily, and it facilitates calcium entry across the plasma membrane in pancreas, placenta, and to a lesser extent stomach and kidney tissue. Furthermore, prostate cancer cells overexpress TRPV6, while benign prostate tissues do not express the protein, implying a role for TRPV6 in malignant growth. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TRPV6 (also designated CaT1) of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 636-725 mapping at the C-terminus of TRPV6 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 636-725 mapping at the C-terminus of TRPV6 of human origin |
| Entrez Gene | 55503 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Western blot analysis of TRPV6 expression in Hep G2 (A), Caco-2 (B), SW480 (C) and DU 145 (D) whole cell lysates.
Application
Western blot analysis of TRPV6 expression in non-transfected: (A) and human TRPV6 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of TRPV6 expression in non-transfected 293T: (A), mouse TRPV6 transfected 293T: (B) and KNRK (C) whole cell lysates.
Application
Western blot analysis of TRPV6 expression in Hep G2 (A), Caco-2 (B), SW480 (C), KNRK (D) and HCT-8 (E) whole cell lysates.| Application Notes | recommended for detection of TRPV6 (also designated CaT1) of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
