Application
Western blot analysis of PGC-1 expression in MDA-MB-435S (A) and Hep G2 (B) whole cell lysates.Application
Western blot analysis of PGC-1 expression in rat kidney tissue extract.Application
Western blot analysis of PGC-1 expression in DU 145 (A) and A-673 (B) nuclear extracts.| Product Name | PGC-1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PPARγ coactivator-1 (PGC-1) of human origin |
| Modification Notes | Transcription factors exert their effects by associating with co-activator or corepressor proteins. The co-activator complexes are thought to be constitutively active, requiring only proper positioning in the genome to initiate transcription. Co-activators include the steroid receptor coactivator (SRC) and CREB binding protein (CBP) families that contain histone acetyltransferase (HAT) activity, which modifies chromatin structure. PPARgamma co-activator-1 (PGC-1) is a transcriptional cofactor of nuclear respiratory factor-1 (NRF-1), PPARbeta, PPARalpha and other nuclear receptors that is induced by exposure to cold temperatures and is involved in regulating thermogenic gene expression, protein uncoupling, and mitochondrial biogenesis. PGC-1 has a low inherent transcriptional activity when it is not bound to a transcription factor. Docking of PGC-1 to PPARgamma stimulates an apparent conformational change that then enables PGC-1 to bind to and assemble into complexes, which include the additional cofactors SRC-1 and CBP/p300, and results in a large increase in transcriptional activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PGC-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PPAR&gamma coactivator-1 (PGC-1) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PPARγ coactivator-1 (PGC-1) of human origin |
| Entrez Gene | 10891 |
|---|
Application
Western blot analysis of PGC-1 expression in MDA-MB-435S (A) and Hep G2 (B) whole cell lysates.
Application
Western blot analysis of PGC-1 expression in rat kidney tissue extract.
Application
Western blot analysis of PGC-1 expression in DU 145 (A) and A-673 (B) nuclear extracts.| Application Notes | recommended for detection of PGC-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
