Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing nuclear staining of neuronal and glial cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of LIN-28 expression in non-transfected 293T: (A), human LIN-28 transfected 293T: (B) and JAR (C) whole cell lysates.Application
Western blot analysis of LIN-28 expression in JAR whole cell lysate.| Product Name | LIN-28 Antibody (H-44) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-44 mapping at the N-terminus of LIN-28 of human origin |
| Modification Notes | LIN-28 is a highly conserved, RNA-binding, cytoplasmic protein. It consists of a cold shock domain and retroviral-type (CCHC) zinc finger motifs that were first identified in Caenorhabditis elegans. LIN-28 controls the timing of events during embryonic development and is readily expressed in embryos, embryonic stem cells and embryonal carcinoma cells. The presence of LIN-28 persists in some adult tissues including cardiac and skeletal muscle. In differentiating myoblasts, LIN-28 increases protein synthesis efficiency and binds to the growth and differentiation factor IGF-II. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of LIN-28 and, to a lesser extent, LIN-28B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-44 mapping at the N-terminus of LIN-28 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-44 mapping at the N-terminus of LIN-28 of human origin |
| Entrez Gene | 79727 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing nuclear staining of neuronal and glial cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of LIN-28 expression in non-transfected 293T: (A), human LIN-28 transfected 293T: (B) and JAR (C) whole cell lysates.
Application
Western blot analysis of LIN-28 expression in JAR whole cell lysate.| Application Notes | recommended for detection of LIN-28 and, to a lesser extent, LIN-28B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
