Application
Western blot analysis of SKI-1 expression in non-transfected 293T: (A), mouse SKI-1 transfected 293T: (B) and Hela (C) whole cell lysates.Application
Western blot analysis of SKI-1 expression in non-transfected 293T: (A), mouse SKI-1 transfected 293T: (B) and Hela (C) whole cell lysates.Application
Western blot analysis of SKI-1 expression in non-transfected 293T: (A), mouse SKI-1 transfected 293T: (B) and IMR-32 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of SKI-1 processed form expression in T98G (A), H4 (B), K-562 (C), Hep G2 (D) and PC-12 (E) whole cell lysates.| Product Name | SKI-1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 753-1052 mapping at the C-terminus of SKI-1 (subtilisin/kexin-isozyme-1) of human origin |
| Modification Notes | Mammalian serine proteases function as proprotein convertases, processing inactive precursors to produce active peptides and proteins. SKI-1 (subtilisin/kexin-isozyme-1) is a type I membrane-bound Ca2+-dependent serine proteinase. SKI-1 is related to bacterial subtilisin and yeast kexin. Subtilisin is an alkaline serine protease produced by Bacillus subtilis 168. Kexin is a prohormone-processing enzyme, which is encoded by the KEX2 gene of the yeast Saccharomyces cerevisiae. SKI-1 is present in most tissues and cells, and it is most concentrated in liver and thyroid tissues. SKI-1 has been shown to cleave the brain-derived neurotrophic factor (BDNF) precursor to produce the mature form of BDNF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of precursor and processed SKI-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 753-1052 mapping at the C-terminus of SKI-1 (subtilisin/kexin-isozyme-1) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 753-1052 mapping at the C-terminus of SKI-1 (subtilisin/kexin-isozyme-1) of human origin |
| Entrez Gene | 8720 |
|---|
Application
Western blot analysis of SKI-1 expression in non-transfected 293T: (A), mouse SKI-1 transfected 293T: (B) and Hela (C) whole cell lysates.
Application
Western blot analysis of SKI-1 expression in non-transfected 293T: (A), mouse SKI-1 transfected 293T: (B) and Hela (C) whole cell lysates.
Application
Western blot analysis of SKI-1 expression in non-transfected 293T: (A), mouse SKI-1 transfected 293T: (B) and IMR-32 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of SKI-1 processed form expression in T98G (A), H4 (B), K-562 (C), Hep G2 (D) and PC-12 (E) whole cell lysates.| Application Notes | recommended for detection of precursor and processed SKI-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
