Application
Western blot analysis of Kininogen HC expression in mouse liver tissue extract.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubuli at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Kininogen HC expression in mouse kidney (A), mouse liver (B), rat kidney (C) and rat liver (D) tissue extracts.| Product Name | Kininogen HC Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 261-330 of Kininogen HC of human origin |
| Modification Notes | Kininogen is a 644 amino acid precursor protein that is expressed by the KNG1 gene and is secreted into blood plasma. Due to alternative splicing events, several Kininogen protein derivatives exist, including Kininogen LC (light chain) and Kininogen HC (heavy chain), both of which are produced from the Kininogen precursor and exhibit different functions throughout the cell. Kininogen HC plays an important role in blood coagulation by helping to ensure that prekallikrein and Factor XI (both of which are involved in blood coagulation) are properly situated for interaction with Factor XII. Additionally, Kininogen HC releases a smaller, active protein known as bradykinin, which plays a role in smooth muscle contraction, induction of hypotension, regulation of blood glucose levels, stimulation of nociceptors and overall mediation of inflammatory responses throughout the cell. In contrast to Kininogen HC, which is involved in blood clotting, Kininogen LC is primarily associated with inhibition of thrombocyte aggregation and also functions as a strong inhibitor of cysteine proteinases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of full length Kininogen precursor and Kininogen heavy chain of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 261-330 of Kininogen HC of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 261-330 of Kininogen HC of human origin |
| Entrez Gene | 3818 |
|---|
Application
Western blot analysis of Kininogen HC expression in mouse liver tissue extract.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubuli at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Kininogen HC expression in mouse kidney (A), mouse liver (B), rat kidney (C) and rat liver (D) tissue extracts.| Application Notes | recommended for detection of full length Kininogen precursor and Kininogen heavy chain of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
