Application
Western blot analysis of TREX-1 expression in HeLa whole cell lysate.Application
Western blot analysis of TREX-1 expression in non-transfected: (A) and mouse TREX-1 transfected: (B) 293T whole cell lysates.| Product Name | TREX-1 Antibody (M-114) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 201-314 mapping at the C-terminus of TREX-1 of mouse origin |
| Modification Notes | TREX-1 (three prime repair exonuclease 1), also known as trophoblast expressed 1, CRV, AGS1, AGS5, DRN3, HERNS or DNase III, is a member of the exonuclease superfamily and belongs to the TREX family. Members of the TREX family are involved in DNA metabolism and repair. TREX-1 is expressed in spleen, liver, thymus, colon, heart, brain and small intestine. It localizes to the nucleus and exists as a homodimer. TREX-1 is an exonuclease and its activity requires magnesium as a cofactor. TREX-1 has a preference for double stranded DNA and functions in the 3’-5’ direction yielding nucleoside 5’-phosphates. TREX-1 also interacts with the SET complex (an endoplasmic reticulum (ER)-associated complex) and participates in granzyme A-mediated apoptosis. Mutations or defects in the gene encoding TREX-1 have been associated with a variety of diseases, including systemic lupus erythematosus, chilblain lupus (CHBL), Aicardi-Goutieres syndrome type 1 (AGS1) and type 5 (AGS5) and autosomal dominant retinal vasculopathy with cerebral leukodystrophy (CRV). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of Three Prime Repair Exonuclease 1 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 201-314 mapping at the C-terminus of TREX-1 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 201-314 mapping at the C-terminus of TREX-1 of mouse origin |
| Entrez Gene | 11277 |
|---|
Application
Western blot analysis of TREX-1 expression in HeLa whole cell lysate.
Application
Western blot analysis of TREX-1 expression in non-transfected: (A) and mouse TREX-1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Three Prime Repair Exonuclease 1 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
