Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing cytoplasmic staining of epidermal cells.Application
Western blot analysis of Cox-1 expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of Cox-1 expression in U-937 whole cell lysate.Application
Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of Cox-1 expression in KNRK (A) and NIH/3T3 (B) whole cell lysates.| Product Name | Cox-1 Antibody (H-62) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 63-124 of Cox-1 of human origin |
| Modification Notes | Prostaglandins are a diverse group of autocrine and paracrine hormones that mediate many cellular and physiologic processes. Prostaglandin H2 (PGH2) is an intermediate molecule in formation of the prostaglandins. Cyclooxygenase-1 (Cox-1) and cyclooxygenase-2 (Cox-2) are prostaglandin synthases that catalyze the formation of PGH2 from arachidonic acid (AA). Cox-1 and Cox-2 are isozymes of prostaglandin-endoperoxidase synthase (PTGS). Cox-1 is constitutively expressed in most tissues and is thought to serve in general “housekeeping” functions. Cox-2 is efficiently induced in migratory cells responding to pro-inflammatory stimuli and is considered to be an important mediator of inflammation. Both enzymes are targets for the nonsteroidal therapeutic anti-inflammatory drugs (NSAIDs). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of Cox-1 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 63-124 of Cox-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 63-124 of Cox-1 of human origin |
| Entrez Gene | 5742 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing cytoplasmic staining of epidermal cells.
Application
Western blot analysis of Cox-1 expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of Cox-1 expression in U-937 whole cell lysate.
Application
Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of Cox-1 expression in KNRK (A) and NIH/3T3 (B) whole cell lysates.| Application Notes | recommended for detection of Cox-1 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
