Application
Western blot analysis of Angiotensin expression in non-transfected: (A) and human Angiotensin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Angiotensin expression in non-transfected: (A) and human Angiotensin transfected: (B) 293 whole cell lysates.Application
Western blot analysis of Angiotensin expression in non-transfected: (A) and human Angiotensin transfected: (B) 293T whole cell lysates.| Product Name | Angiotensin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Angiotensin precursor of human origin |
| Modification Notes | Angiotensin is formed from a precursor, angiotensinogen, which is produced by the liver and found in the a-globulin fraction of plasma. The lowering of blood pressure is a stimulus to secretion of Renin by the kidney into the blood. Renin cleaves from angiotensinogen a terminal decapeptide, Angiotensin I (Ang I). This is further altered by the enzymatic removal of a dipeptide to form Angiotensin II (Ang II). Screening a panel of human-mouse somatic cell hybrids confirmed the assignment of the AGT locus to human chromosome 1. Angiotensin, an octapeptide hormone, is an important physiological effector of blood pressure and volume regulation through vasoconstriction, aldosterone release, sodium uptake and thirst stimulation. It has been shown that mechanical stress causes release of Angiotensin from cardiac myocytes and that Angiotensin acts as an initial mediator of the hypertrophic response. Angiotensin treatment also stimulates phosphorylation of Shc, FAK and MAP kinases and induces MKP-1, indicating stimulation of growth factor pathways. Angiotensin stimulation through AT1 has been shown to activate the JAK/Stat pathway involving a direct interaction between JAK2 and AT1 as demonstrated by co-immunoprecipitation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Angiotensin I and Angiotensin II of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Angiotensin precursor of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Angiotensin precursor of human origin |
| Entrez Gene | 183 |
|---|
Application
Western blot analysis of Angiotensin expression in non-transfected: (A) and human Angiotensin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Angiotensin expression in non-transfected: (A) and human Angiotensin transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of Angiotensin expression in non-transfected: (A) and human Angiotensin transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Angiotensin I and Angiotensin II of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
