Application
Immunofluorescence staining of methanol-fixed untransfected (A) and human MEK-3 transfected HEK 293T cells (B).Application
Western blot analysis of MEK-3 expression in non-transfected: (A) and human MEK-3 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of MEK-3 expression in non-transfected: (A) and human MEK-3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of MEK-3 expression in Jurkat whole cell lysate.Application
Western blot analysis of MEK-3 expression in non-transfected: (A) and human MEK-3 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human muscle tissue showing cytoplasmic staining.Application
Western blot analysis of MEK-3 expression in NIH/3T3 (A) and Src-3T3 (B) whole cell lysates.| Product Name | MEK-3 Antibody (I-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the N-terminus of MEK-3 of human origin |
| Modification Notes | A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of MEK-3 and, to a lesser extent, MEK-6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the N-terminus of MEK-3 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the N-terminus of MEK-3 of human origin |
| Entrez Gene | 5606 |
|---|
Application
Immunofluorescence staining of methanol-fixed untransfected (A) and human MEK-3 transfected HEK 293T cells (B).
Application
Western blot analysis of MEK-3 expression in non-transfected: (A) and human MEK-3 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of MEK-3 expression in non-transfected: (A) and human MEK-3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of MEK-3 expression in Jurkat whole cell lysate.
Application
Western blot analysis of MEK-3 expression in non-transfected: (A) and human MEK-3 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human muscle tissue showing cytoplasmic staining.
Application
Western blot analysis of MEK-3 expression in NIH/3T3 (A) and Src-3T3 (B) whole cell lysates.| Application Notes | recommended for detection of MEK-3 and, to a lesser extent, MEK-6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
