Application
Western blot analysis of PI 3-kinase p110α expression in Jurkat whole cell lysate.| Product Name | PI 3-kinase p110α Antibody (H-201) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 189-390 mapping near the N-terminus of PI 3-kinase p110α of human origin |
| Modification Notes | Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85å and p85∫), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110å and p110∫ interact with p85å, and p110å has also been shown to interact with p85∫ in vitro. p110∂ expression is restricted to white blood cells. It has been shown to bind p85å and ∫, but it apparently does not phosphorylate these subunits. p110∂ seems to have the capacity to autophosphorylate. p110© does not interact with the p85 subunits. It has been shown to be activated by å and ∫© heterotrimeric G proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PI 3-kinase p110α of mouse, rat, human and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 189-390 mapping near the N-terminus of PI 3-kinase p110&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 189-390 mapping near the N-terminus of PI 3-kinase p110α of human origin |
| Entrez Gene | 5290 |
|---|
Application
Western blot analysis of PI 3-kinase p110α expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of PI 3-kinase p110α of mouse, rat, human and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
