Application
Western blot analysis of eIF4G expression in non-transfected 293T: (A), mouse eIF4G transfected 293T: (B), Jurkat (C) and NIH/3T3 (D) whole cell lysates.Application
Immunofluorescence staining of normal mouse liver frozen section showing nuclear staining.| Product Name | eIF4G Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of eIF4G of human origin |
| Modification Notes | The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4G, eIF4E, and eIF4A. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures (1,2). eIF4E binds to the mRNA “cap” during an early step in the initiation of protein synthesis (3,4). eIF4A acts as an ATP-dependent RNA helicase (5,6). eIF4G acts as a bridge between eIF4E, eIF4A, and the eIF3 complex (7,8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of eIF4G of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of eIF4G of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of eIF4G of human origin |
| Entrez Gene | 1981 |
|---|
Application
Western blot analysis of eIF4G expression in non-transfected 293T: (A), mouse eIF4G transfected 293T: (B), Jurkat (C) and NIH/3T3 (D) whole cell lysates.
Application
Immunofluorescence staining of normal mouse liver frozen section showing nuclear staining.| Application Notes | recommended for detection of eIF4G of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
