Application
Western blot analysis of Neurogranin expression in rat brain tissue extract.| Product Name | Neurogranin Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-50 mapping at the N-terminus of Neurogranin of human origin |
| Modification Notes | Neurogranin (formerly designated p17, also known as RC3 and BICKS) is a neuron-specific substrate for protein kinase C (PKC) (1). Neurogranin is a postsynaptic protein that is highly enriched in brain, with restricted expression in the cortex, striatum, hippocampus, thalamus, hypothalamus and olfactory bulb nuclei (2,3). Neurogranin binds calmodulin at low levels of calcium, thereby regulating calmodulin-dependent nitric oxide synthase (4). Conversely, nitric oxide modifies neurogranin, reducing its ability to bind calmodulin or to be phosphorylated by PKC (5). This phosphorylation site domain is adjacent to the predicted calmodulin-binding region (6,7) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Neurogranin of mouse, rat and human origin by WB, IP, IF and ELISA may cross-react with Neuromodulin also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-50 mapping at the N-terminus of Neurogranin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-50 mapping at the N-terminus of Neurogranin of human origin |
| Entrez Gene | 4900 |
|---|
Application
Western blot analysis of Neurogranin expression in rat brain tissue extract.| Application Notes | recommended for detection of Neurogranin of mouse, rat and human origin by WB, IP, IF and ELISA may cross-react with Neuromodulin also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
