Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing membrane and cytoplasmic localization.Application
Western blot analysis of EP3 expression in non-transfected control (A) and EP3 siRNA transfected (B) HeLa cells. Blot probed with EP3 (H-200): . β Tubulin (D-10): used as specificity and loading control.Application
Western blot analysis of EP3 expression in JAR whole cell lysate.| Product Name | EP3 Antibody (H-200) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-200 of EP3 of human origin |
| Modification Notes | Prostaglandin E2, a member of the autacoid family of lipid mediators, is a major renal cyclooxygenase product of arachidonic acid metabolism (1–3). Prostaglandin E2 binds to four G protein-coupled E-prostanoid receptors, designated EP1, EP2, EP3 and EP4 (1–3). The expression and function of the prostaglandin E2 receptors have been highly characterized in kidney (2,3). EP1, which is predominantly expressed in the collecting duct, couples to Gq proteins to inhibit sodium absorption and increase in intracellular calcium, which act as second messengers (2–4). EP2 is coupled to Gs proteins, which stimulate adenylyl cyclase (4,5). EP2 has the lowest expression in kidney, but EP2 knockout mice exhibit salt-sensitive hypertension, which suggests a role for EP2 in salt excretion (2,3). EP3 is expressed in renal vessels, thick ascending limb and collecting duct (2,3). EP3 has at least 6 alternative splice variants that couple to Gi proteins to inhibit cAMP, which subsequently inhibit sodium and water transport (2–4,6). In uterus, EP3 induces the contraction of uterine smooth muscles (7). EP4 is expressed in glomerulus and collecting duct. It couples to Gs proteins, which stimulate adenylyl cyclase and regulate glomerular tone and renal renin release (2–5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of all EP3 isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-200 of EP3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-200 of EP3 of human origin |
| Entrez Gene | 5733 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing membrane and cytoplasmic localization.
Application
Western blot analysis of EP3 expression in non-transfected control (A) and EP3 siRNA transfected (B) HeLa cells. Blot probed with EP3 (H-200): . β Tubulin (D-10): used as specificity and loading control.
Application
Western blot analysis of EP3 expression in JAR whole cell lysate.| Application Notes | recommended for detection of all EP3 isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
