Application
Western blot analysis of p16 expression in H69AR whole cell lysate.Application
Western blot analysis of p16 expression in 3T3-L1 (A) and mouse Lac-Z (B) whole cell lysates.Application
Western blot analysis of p16 expression in SHP-77 whole cell lysate.Application
Western blot analysis of p16 expression in Saos-2 (A) and HeLa (B) cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | p16 Antibody (N-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of p16 of human origin |
| Modification Notes | The progression of cells through the cell cycle is regulated by a family of protein kinases known as cyclin-dependent kinases (Cdks). The sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle. The cyclins function as differentially expressed positive regulators of Cdks. Negative regulators of the cycle include the p53-inducible protein p21 (also designated WAF1 or Cip1), Kip1 p27 and p16. The complexes formed by Cdk4 and the D-type cyclins have been strongly implicated in the control of cell proliferation during the G1 phase. It has been shown that p16 binds to Cdk4 and inhibits the catalytic activity of the Cdk4/cyclin D complex. Moreover, the gene encoding p16 exhibits a high frequency of homozygous deletions and point mutations in established human tumor cell lines. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of p16 of human and, to a lesser extent, mouse origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of p16 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of p16 of human origin |
| Entrez Gene | 1029 |
|---|
Application
Western blot analysis of p16 expression in H69AR whole cell lysate.
Application
Western blot analysis of p16 expression in 3T3-L1 (A) and mouse Lac-Z (B) whole cell lysates.
Application
Western blot analysis of p16 expression in SHP-77 whole cell lysate.
Application
Western blot analysis of p16 expression in Saos-2 (A) and HeLa (B) cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of p16 of human and, to a lesser extent, mouse origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
