Application
Western blot analysis of recombinant GST fusion protein.Application
Western blot analysis of recombinant GST under reducing conditions.| Product Name | GST Antibody (110-218) |
|---|---|
| Antibody Type | Tags Antibodies |
| Antigen Alias | epitope corresponding to amino acids 110-218 mapping at the C-terminus of GST of Schistosoma japonicum origin |
| Modification Notes | Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coli are in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GST fusion proteins and glutathione-S-transferase (GST) of Schistosoma japonicum origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 110-218 mapping at the C-terminus of GST of Schistosoma japonicum origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 110-218 mapping at the C-terminus of GST of Schistosoma japonicum origin |
Application
Western blot analysis of recombinant GST fusion protein.
Application
Western blot analysis of recombinant GST under reducing conditions.| Application Notes | recommended for detection of GST fusion proteins and glutathione-S-transferase (GST) of Schistosoma japonicum origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
