Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of BMPR-IB expression in DU 145 (A), LNCaP (B) and PC-3 (C) whole cell lysates.| Product Name | BMPR-IB Antibody (H-44) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 15-58 of BMPR-IB of human origin |
| Modification Notes | Members of the transforming growth factor b superfamily bind to a pair of transmembrane proteins, known as receptor types I and II, which contain serine/threonine kinases and associate to form a signaling complex (1). Two type I receptors have been characterized, BMPR-IA (also designated SKR5, ALK-3, and BRK-1) and BMPR-IB (also designated ALK-6 and SKR 6), that bind to bone morphogenetic proteins (BMP)-2, BMP-4, and osteogenic protein (OP)-1 (also designated BMP-7) (1–4). BMPR-IA and BMPR-IB are both expressed in human glioma cell lines (3). The type II receptor, BMPR-II, efficiently binds to OP-1 and BMP-2 and weakly binds BMP-4, and it is widely expressed in different tissues, including brain (1,5,6). The BMP receptor family members are thought to mediate distinct effects on gene expression, cell differentiation, and morphogenesis in a dose dependent fashion (1,5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of BMPR-IB of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 15-58 of BMPR-IB of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 15-58 of BMPR-IB of human origin |
| Entrez Gene | 658 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of BMPR-IB expression in DU 145 (A), LNCaP (B) and PC-3 (C) whole cell lysates.| Application Notes | recommended for detection of BMPR-IB of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
