Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic staining of cells in tubules.Application
Western blot analysis of PTH-rP expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of PTH-rP expression in 3T3-L1 (A), NIH/3T3 (B) and MCF7 (C) whole cell lysates.| Product Name | PTH-rP Antibody (H-137) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 41-177 mapping at the C-terminus of parathyroid hormone-related protein (PTN-rP) of human origin |
| Modification Notes | Parathyroid hormone (PTH), which is also designated parathyrin, is an 84 amino acid single chain peptide that functions to regulate calcium metabolism by raising blood levels of calcium through various mechanisms. PTH stimulates bone formation to increase bone mass and strength in rats and humans. Within the PTH molecule, the essential activity is associated with the first 34 amino acids at the amino-terminus of the molecule. Parathyroid hormone-related protein (PTH-rP) is an autocrine factor that is structurally related to PTH yet, unlike PTH, which is synthesized only by the parathyroid cells, PTH-rP is synthesized by several cell types. PTH-rP regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. Isolated from the culture medium of a human lung cancer cell line, PTH-rP produces PTH-like effects that are characterized as humoral hypercalcemia of malignancy. Both PTH and PTH-rP are regulated by vitamin D and steroid hormones, and both preferentially bind to specific PTH/PTH-rP receptors, then activate adenylate cyclase or PLC ∫ via PKC activation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of precursor and mature PTH-rP and osteostatin active peptide of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 41-177 mapping at the C-terminus of parathyroid hormone-related protein (PTN-rP) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 41-177 mapping at the C-terminus of parathyroid hormone-related protein (PTN-rP) of human origin |
| Entrez Gene | 5744 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic staining of cells in tubules.
Application
Western blot analysis of PTH-rP expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of PTH-rP expression in 3T3-L1 (A), NIH/3T3 (B) and MCF7 (C) whole cell lysates.| Application Notes | recommended for detection of precursor and mature PTH-rP and osteostatin active peptide of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
