Application
Immunofluorescence staining of methanol-fixed untransfected (A) and human PAK6 transfected HEK 293T cells (B).Application
Western blot analysis of PAK6 expression in SK-N-MC whole cell lysate.Application
Western blot analysis of PAK6 expression in non-transfected: (A) and human PAK6 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PAK6 expression in non-transfected: (A) and human PAK6 transfected: (B) 293T whole cell lysates.| Product Name | PAK6 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 71-370 mapping within an internal region of PAK6 of human origin |
| Modification Notes | The p21(CDKN1A)-activated kinases (PAKs) are serine/threonine protein kinases that bind to activated small GTPases, including Cdc42 and Rac, and influence transcription, cell morphology (cytoskeleton rearrangement), motility, and apoptosis. PAK family members contain an amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK6 protein cotranslocates into the nucleus with androgen receptor, which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development, in response to androgen. PAK6 transcripts are present at high levels in brain and testis, with lower levels in multiple tissues including prostate and breast. The human PAK6 gene maps to chromosome 15q14. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PAK6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 71-370 mapping within an internal region of PAK6 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 71-370 mapping within an internal region of PAK6 of human origin |
| Entrez Gene | 56924 |
|---|
Application
Immunofluorescence staining of methanol-fixed untransfected (A) and human PAK6 transfected HEK 293T cells (B).
Application
Western blot analysis of PAK6 expression in SK-N-MC whole cell lysate.
Application
Western blot analysis of PAK6 expression in non-transfected: (A) and human PAK6 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PAK6 expression in non-transfected: (A) and human PAK6 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PAK6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
