Application
Immunopero-xidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic, centrosomal and nuclear localization.Application
Immunofluo-rescence staining of methanol-fixed HeLa cells showing cytoplasmic, centrosomal and nuclear localization.Application
Western blot analysis of p70 S6 kinase α phosphorylation expression in untreated (A) and EGF treated (B) MCF7 whole cell lysates.| Product Name | p-p70 S6 kinase α Antibody (Ser 424) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 424 phosphorylated p70 S6 kinase α isoform Alpha II of human and mouse origin, and Ser 447 phosphorylated p70 S6 kinase α of human, mouse and rat origin of m, r and h origin by WB, IP, IF and IHC(P) |
| Modification | Phosphoralated |
| Modification Notes | In studies to elucidate key regulatory pathways in signal transduction, several protein serine/threonine (Ser/Thr) kinases have been identified. Included among such kinases are two distinct families of 40S ribosomal protein S6 Ser/Thr kinases present in somatic animal cells, designated p70 S6 kinase and p90 Rsk kinase. p90 Rsk kinase is maximally activated within minutes of addition of growth factors or phorbol ester to cultured cells followed by activation of p70 S6 kinase. Both enzymes are regulated by serine/threonine phosphorylation, suggesting that specific kinases may exist upstream in the signaling pathway that regulate these kinases. In fact, evidence suggests that one such family of activating enzymes includes the members of the ERK MAP kinase family. The ERK MAP kinases are, in turn, regulated by phosphorylation at threonine and tyrosine residues by a protein kinase designated MEK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 424 phosphorylated p70 S6 kinase &alpha isoform Alpha II of human and mouse origin and Ser 447 phosphorylated p70 S6 kinase &alpha of human mouse and rat origin of m r and h origin by WB IP IF and IHC(P) |
|---|---|
| Tissue Specificity | recommended for detection of Ser 424 phosphorylated p70 S6 kinase α isoform Alpha II of human and mouse origin, and Ser 447 phosphorylated p70 S6 kinase α of human, mouse and rat origin of m, r and h origin by WB, IP, IF and IHC(P) |
| Entrez Gene | 6198 |
|---|
Application
Immunopero-xidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic, centrosomal and nuclear localization.
Application
Immunofluo-rescence staining of methanol-fixed HeLa cells showing cytoplasmic, centrosomal and nuclear localization.
Application
Western blot analysis of p70 S6 kinase α phosphorylation expression in untreated (A) and EGF treated (B) MCF7 whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
