Application
Western blot analysis of Ly-6A/E expression in BW5147 whole cell lysate.| Product Name | Ly-6A/E Antibody (M-86) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 27-112 mapping within an internal region of Ly-6A/E of mouse origin |
| Modification Notes | The activity of natural killer (NK) cells is regulated by members of multiple receptor families that recognize class I MHC molecules, such as the killer cell inhibitory receptor/leukocyte immunoglobulin-like receptor (KIR/LIR) family and the C-type lectin superfamily. The Ly-6 alloantigens represent a family of phosphatidylinositol anchored proteins that play a role in the process of T lymphocyte activation. Ly-6 expression is commonly induced on T and B lymphocytes after activation by mitogens. Ly-6A/E is a phosphatidylinositol-linked transmembrane member of the Ly-6 family that is produced in humans after 4 weeks of age. ©-interferon and TNF regulate Ly-6A/E at the level of transcription and mRNA stabilization in certain thymocytes and T-cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ly-6A/E, and to a lesser extent, with other Ly-6 family members of mouse origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 27-112 mapping within an internal region of Ly-6A/E of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 27-112 mapping within an internal region of Ly-6A/E of mouse origin |
| Entrez Gene | 110454 |
|---|
Application
Western blot analysis of Ly-6A/E expression in BW5147 whole cell lysate.| Application Notes | recommended for detection of Ly-6A/E, and to a lesser extent, with other Ly-6 family members of mouse origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
