Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Antibodies tested include PKN (N-19): (A), PKN (C-19): (B) and PKN (H-234): (C,D).| Product Name | PKN Antibody (H-234) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 569-803 mapping at the C-terminus of PKN of human origin |
| Modification Notes | Rho, the Ras-related small GTPase, is responsible for the regulation of actin-based cytoskeletal structures including stress fibers, focal adhesions and the contractile ring apparatus. Rho proteins act as molecular switches which are able to turn cytokinesis on and off. Although little is know about signaling downstream of Rho, several proteins have been implicated as Rho effectors. Protein kinase N (PKN) is a fatty acid-activated serine/threonine kinase whose catalytic domain exhibits homology with that of the PKC family. PKN associates with Rho via its amino terminus, is activated in a GTP-dependent manner and phosphorylates the head-rod domain of neurofilament protein. A second protein, rhophilin, exhibits 40% sequence identity with the amino terminal Rho binding domain. The enzymatic activity of rhophilin has not been demonstrated and it is possible that it acts through the recruitment of cytoskeletal components that initiate a kinase signaling cascade. Citron interacts specifically with active Rho and Rac1 but not Cdc42. Citron exhibits a distinctive protein organization and little homology with the Rho binding domains of PKN and rhophilin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PKN of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 569-803 mapping at the C-terminus of PKN of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 569-803 mapping at the C-terminus of PKN of human origin |
| Entrez Gene | 5585 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Antibodies tested include PKN (N-19): (A), PKN (C-19): (B) and PKN (H-234): (C,D).| Application Notes | recommended for detection of PKN of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
