| Product Name | Kar2 Antibody (y-115) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 568-682 mapping at the C-terminus of Kar2 of Saccharomyces cerevisiae origin |
| Modification Notes | The yeast KAR2 gene was isolated by complementation of a mutation that blocks nuclear fusion (1). The predicted KAR2 protein sequence is most structurally homologous to mammalian BiP/GRP78 (1). The endoplasmic reticulum (ER) of mammalian cells contains a protein BiP that is believed to assist in the folding of secretory and transmembrane proteins (2). In yeast, Kar2 function is required for translocation of soluble proteins into the ER at a stage beyond the initial nascent chain-membrane association (3). Deficiencies in Kar2 may cause generalized failure of protein folding in the ER, leading to pleiotropic effects on cellular metabolism (2). The unfolded protein response (UPR) is a signal transduction pathway induced by a variety of ER stresses and functions to maintain homeostasis of the cellular membrane in eukaryotes (4,5). Additionally, Kar2 directly regulates the UPR by the interaction the transmembrane protein kinase/ribonuclease, Ire1, which transmits a signal from the ER to the nucleus (4,5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Kar2 of Saccharomyces cerevisiae origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 568-682 mapping at the C-terminus of Kar2 of Saccharomyces cerevisiae origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 568-682 mapping at the C-terminus of Kar2 of Saccharomyces cerevisiae origin |
| Application Notes | recommended for detection of Kar2 of Saccharomyces cerevisiae origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
