Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing cytoplasmic staining of epidermal cells.Application
Western blot analysis of desmoplakin I/II expression in HeLa whole cell lysate.Application
Immunofluores-cence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | desmoplakin I/II Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 79-300 mapping near the N-terminus of desmoplakin I of human origin |
| Modification Notes | Desmosomes are major cell adhesion junctions that are particularly prominent in the epidermis and in cardiac tissue and are important for the rigidity and strength of the cell (1). The desmosome consists of several proteins, of which desmoplakin is the most abundant (1). Desmoplakin plays an important role in the attachment of the filaments to the desmosome (1). Specifically, desmoplakin interacts with plakophilin 1 (PKP1), PKP2 or PKP3, or combinations thereof, to selectively recruit plakophilins to desmosomal plaques (2). Desmoplakin has also been shown to function as a transglutaminase substrate in vitro, suggesting that it may participate in cell adhesion at the intraepidermal level (3). Desmoplakin exists as a two-stranded coiled coil structure (4,5). Alternative splicing gives rise to two isoforms, desmoplakin I and II, which differ by 600 amino acids (4). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of desmoplakin I/II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 79-300 mapping near the N-terminus of desmoplakin I of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 79-300 mapping near the N-terminus of desmoplakin I of human origin |
| Entrez Gene | 1832 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing cytoplasmic staining of epidermal cells.
Application
Western blot analysis of desmoplakin I/II expression in HeLa whole cell lysate.
Application
Immunofluores-cence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of desmoplakin I/II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
