Application
Western blot analysis of TLR4 expression in WEHI-231 whole cell lysate.Application
Western blot analysis of TLR4 expression in Jurkat (A), HEL 92.1.7 (B), HL-60 (C), AML-193 (D), CCRF-CEM (E) and JAR (F) whole cell lysates.| Product Name | TLR4 Antibody (M-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 339-638 mapping within an N-terminal extracellular domain of TLR4 of mouse origin |
| Modification Notes | Six human homologs of the Drosophila Toll receptor were initially identified based on their sequence similarities and designated toll-like receptors (TLR). Toll receptors are involved in mediating dorsoventral polarization in the developing Drosophila embryo and also participate in the host immunity. The TLR family of proteins are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transduction. TLR1, as well as the other TLR family members, are type I transmembrane receptors that characteristically contain an extracellular domain consisting of several leucine-rich regions along with a single cytoplasmic Toll/IL-1R-like domain. TLR2 and TLR4 are activated in response to lipopolysacchride (LPS) stimulation, which results in the activation and translocation of NFkB and suggests that these receptors are involved in mediating inflammatory responses. Expression of TLR receptors is highest in peripheral blood leukocytes, macro-phages, and monocytes. TLR6 is highly homologous to TLR1, sharing greater than 65% sequence identity, and, like other members of TLR family, it induces NFkB signaling upon activation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TLR4 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 339-638 mapping within an N-terminal extracellular domain of TLR4 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 339-638 mapping within an N-terminal extracellular domain of TLR4 of mouse origin |
| Entrez Gene | 7099 |
|---|
Application
Western blot analysis of TLR4 expression in WEHI-231 whole cell lysate.
Application
Western blot analysis of TLR4 expression in Jurkat (A), HEL 92.1.7 (B), HL-60 (C), AML-193 (D), CCRF-CEM (E) and JAR (F) whole cell lysates.| Application Notes | recommended for detection of TLR4 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
