Application
Western blot analysis of BAF53 expression in IMR-32 (A), HeLa (B) and SH-SY5Y (C) nuclear extracts.Application
Western blot analysis of BAF53 expression in non-transfected: (A) and mouse BAF53 transfected: (B) 293T whole cell lysates.| Product Name | BAF53 Antibody (H-125) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 47-171 mapping near the N-terminus of BAF53 of human origin |
| Modification Notes | The SWI/SNF complex regulates gene expression via ATP-dependent chromatin remodeling. Brm (SNF2-a), Brg-1 (SNF2-b), Ini1 (integrase interactor 1, SNF5), BAF53 (ARPn∫), BAF57, BAF155 (SRG3), and BAF170 make up the functional core. BAF53 homologues from yeast to humans contain a conserved N-terminal motif, which contains residues at serine 2 and tyrosine 6, which play important roles in BAF53 activity. The BAF53 protein shuttles between the nucleus and cytoplasm. BAF53 also forms a complex with TIP49 and TIP48, which mediates c-Myc oncogenic activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of BAF53 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 47-171 mapping near the N-terminus of BAF53 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 47-171 mapping near the N-terminus of BAF53 of human origin |
Application
Western blot analysis of BAF53 expression in IMR-32 (A), HeLa (B) and SH-SY5Y (C) nuclear extracts.
Application
Western blot analysis of BAF53 expression in non-transfected: (A) and mouse BAF53 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of BAF53 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
