Application
Western blot analysis of LAT2 expression in Hep G2 whole cell lysate.| Product Name | LAT2 Antibody (P-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of LAT2 of mouse, rat, human and zebrafish origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | L-amino acid transporter protein-2 (LAT2), a non-glycosylated membrane protein, complexes with CD98 to contribute to readsorption of neutral amino acids in renal epithelia and blood-tissue barriers. The gene encoding LAT2 is expressed primarily in the kidney, but also to a lesser extent in placenta, brain, liver, spleen, skeletal muscle, heart, small intestine, and lung. Transfection with the antisense sequence of LAT2 suggests that LAT2 expression plays a major role in net basolateral efflux of cysteine, and points to LAT2 as a candidate gene to modulate cysteine reabsorption. In addition, the CD98/LAT2 heterodimer associates with Integrin ∫1 in intestinal epithelial cells, where ligand binding to CD98 and another cell surface molecule, ICAM-1 differentially regulates LAT2 activity, suggesting a novel mechanism by which events like cell adhesion may affect amino acid transport activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of LAT2 of mouse rat human and zebrafish origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of LAT2 of mouse, rat, human and zebrafish origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 23428 |
|---|
Application
Western blot analysis of LAT2 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
