Application
Western blot analysis of LTA Antibody (Center) (Cat. #AP8509c) in MDA-MB231 cell line lysates (35ug/lane). LTA (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with LTA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of HepG2 cells using LTA Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | LTA Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LTA; TNFB; TNFSF1; Lymphotoxin-alpha; TNF-beta; Tumor necrosis factor ligand superfamily member 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TNFB TNFSF1 |
|---|---|
| Alternative Names | LTA TNFB TNFSF1 Lymphotoxin-alpha TNF-beta Tumor necrosis factor ligand superfamily member 1 |
| Molecular Weight(MW) | 22297 Da |
| Function | Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo |
| Tissue Specificity | This LTA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52~81 amino acids from the Center region of human LTA. |
| Cellular Localization | Secreted. Membrane. Note=The homotrimer is secreted. The heterotrimer is membrane-associated |
| Entrez Gene | 4049 |
|---|
Application
Western blot analysis of LTA Antibody (Center) (Cat. #AP8509c) in MDA-MB231 cell line lysates (35ug/lane). LTA (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with LTA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of HepG2 cells using LTA Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
