Application
Western blot analysis of ST8Sia IV expression in non-transfected: (A) and human ST8Sia IV transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ST8Sia IV expression in mouse spleen (A) and rat spleen (B) tissue extracts.Application
Western blot analysis of ST8Sia IV expression in non-transfected: (A) and human ST8Sia IV transfected: (B) 293 whole cell lysates and mouse brain tissue extract (C).| Product Name | ST8Sia IV Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 21-90 mapping near the N-terminus of ST8Sia IV of human origin |
| Modification Notes | ST8Sia IV (ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase IV), also known as PST, PST1 or SIAT8D, is a 359 amino acid single-pass type II membrane protein that localizes to the membrane of the Golgi apparatus. Highly expressed in heart, thymus and spleen, as well as fetal lung, brain and kidney, ST8Sia IV functions to catalyze the polycondensation of alpha-2,8-linked sialic acid, an event that is required for the synthesis of polysialic acid (PSA). PSA is an important regulator of neuronal plasticity and is present in embryonic brain tissue, where it interacts with NCAM (neural cell adhesion molecule) and plays a crucial role in fetal brain development. Defects in the gene encoding ST8Sia IV are associated with idiopathic pancreatitis, schizophrenia and tumor formation/metastasis. ST8Sia IV exists as multiple isoforms produced by alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ST8Sia IV of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-90 mapping near the N-terminus of ST8Sia IV of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-90 mapping near the N-terminus of ST8Sia IV of human origin |
| Entrez Gene | 7903 |
|---|
Application
Western blot analysis of ST8Sia IV expression in non-transfected: (A) and human ST8Sia IV transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ST8Sia IV expression in mouse spleen (A) and rat spleen (B) tissue extracts.
Application
Western blot analysis of ST8Sia IV expression in non-transfected: (A) and human ST8Sia IV transfected: (B) 293 whole cell lysates and mouse brain tissue extract (C).| Application Notes | recommended for detection of ST8Sia IV of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
